SpectraMax iD3 多功...

SpectraMax iD3 多功能酶标仪参数指标

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多功能酶标仪SpectraMax iD3  核心参数

  • 吸光度线性:0-2 0.

  • 精确度值:<±0.010 OD

  • 波长检测速度:光吸收96孔25秒

  • 吸光值范围:230-1000 nm

  • 制作曲线类型:参照软件

  • 实验方法:终点法、动力法、扫描法

  • 自动程度:全自动酶标仪

  • 仪器种类:多通道酶标仪

  • 检测器:PMT photodiode

  • 波长范围:光吸收230-1000 nm;荧光250-850 nm;化学发光300-850 nm

  • 仪器功能:多功能

  • 产地类别:进口仪器


General specifications

Dimensions (in.)15.79 (H) x 20.94 (W) x 23.54 (D)
Dimensions (cm)40.1 (H) x 53.2 (W) x 59.8 (D)
Weight88.1 lbs. (40 kg)
Power requirements100–240 VAC, 2 A, 50/60 Hz
Robotic compatibleYes

General performance

Plate formats6 to 384 wells 
Light sourceXenon flash lamp
Reading capabilitiesMicroplates, cuvettes (via adapter)
DetectorsPhoto Multiplier Tube and Photodiode
ShakingOrbital, Infinity
Temp. control5°C above ambient to 65°CŒ
Temp. uniformity± 0.75°C
Temp. accuracy±1°C at 37°C set point
Spectral scanningAbs, FI, Lum
Endpoint readingAbs, FI, Lum
Kinetic readingAbs, FI, Lum
Well scanningOver 20 by 20 in all modes
Wavelength selection1.0 nm increments

Standard read times (minutes:seconds)


96 wells

384 wells

Absorbance0:251:25
Fluorescence intensity0:170:53
Luminescence0:261:01

Absorbance photometric performance

Wavelength range230–1000 nm
Wavelength bandwidth4.0 nm
Wavelength accuracy± 2.0 nm
Wavelength repeatability± 1.0 nm
Photometric range0–4.0 OD
Photometric resolution0.001 OD
Photometric accuracy< ±0.010 OD ±1.0%, 0–3 OD
Photometric precision< ±0.003 OD ±1.0%, 0–3 OD
Stray light< 0.05% @ 230 nm

Fluorescence intensity performance

Wavelength range250–850 nm
Wavelength selection1.0 nm increments
Dynamic range> 6 decades

Optimized Top sensitivity (fluorescein)

96 wells1 pM
384 wells1 pM

Optimized Bottom sensitivity (fluorescein)

96 wells2 pM
384 wells2.5 pM

Luminescence performance

Wavelength range300–850 nm
Wavelength selectionChoice of simultaneous detection of all wavelengths or selection in 1.0 nm increments
Dynamic range> 7 decades
Cross-talk< 0.1% in white 96- and < 0.2% in 384-well microplates

Optimized Sensitivity (ATP-Glow)

96 wells2 pM
384 wells4 pM

Injector system with SmartInject Technology (optional)

Injectors2
Read modesAbsorbance, fluorescence, luminescence
Dispense accuracy± 5% at 100 μL
Dispense precision

CV ≤ 2% at 100 μL

Dead volumeInjector tubing: 250 μL
< 10 μL with Reverse Prime function
Detection limit20 amol ATP (“Flash“ luminescence using Promega ENLITEN® ATP Assay System)