ACK1Antibody(N-term)

ACK1 Antibody(N-term)
Cat.#: T2674
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: Q07912
Mol Weight: 114kDa
Size: 100ug

Description
ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.

Recommended Applications
WB, IHC, FC, ELISA

Applications and Recommended Dilution Factors
WB: 1:50~100
IHC: 1:50~100
FC: 1:10~50
ELISA: 1:1,000

Species Reactivity
Human

Products Data  

 

Western blot analysis of ACK1 Antibody (N-term) in K562 cell line lysates (35ug/lane). ACK1 (arrow) was detected using the purified Pab.
Western blot analysis of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACK1 gene (Lane 2) (60 KD recombinant protein).
Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with ACK1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specificity
This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17~47 amino acids from the N-terminal region of human ACK1.

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Alternative Names
TNK2 antibody, ACK antibody, ACK1 antibody, FLJ44758 antibody, FLJ45547 antibody, p21cdc42Hs antibody, Activated CDC42 kinase 1 antibody, Tyrosine kinase non-receptor protein 2 antibody

Description References
Yokoyama, N., et al., J. Biol. Chem. 278(48):47713-47723 (2003).
Manser, E., et al., Nature 363(6427):364-367 (1993).