ALDH4A1 Antibody(Center)
Cat.#: T2825
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P30038
Mol Weight: 62kDa
Size: 100ug

Description
ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline.

Recommended Applications
WB, IHC, ELISA

Applications and Recommended Dilution Factors
WB: 1:50~100
IHC: 1:50~100
ELISA: 1:1,000

Species Reactivity
Human

Products Data 

 

Western blot analysis of anti-ALDH4A1 Antibody (Center) in HepG2 cell line lysates (35ug/lane). ALDH4A1(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH4A1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specificity
This ALDH4A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294~324 amino acids from the Center region of human ALDH4A1.

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Alternative Names
ALDH4A1 antibody, ALDH4 antibody, DKFZp779M035 antibody, P5CD antibody, P5CDh antibody, Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial antibody, Aldehyde dehydrogenase family 4 member A1 antibody

Description References
Yoon,K.A., J. Hum. Genet. 49 (3), 134-140 (2004)
Geraghty,M.T., Hum. Mol. Genet. 7 (9), 1411-1415 (1998)