CarboPac SA10 Column for Fast, High-Resolution Mono- and Disaccharide Analysis
The Thermo Scientific Dionex CarboPac SA10 column line has been expanded to include a new 2 × 250 mm microbore column. This microbore column complements the already available standard-bore Dionex CarboPac SA10 (4 × 250 mm) Column. The new 2mm format is optimally suitable for interfacing with Mass Spectrometric Detectors, as well as Pulsed Amperometric Detectors.
The Dionex CarboPac SA10 anion-exchange column is designed for fast chromatographic analysis ofsimple sugars in biofuel and food samples. The fast, efficient separations of mono- and disaccharides are achieved without compromising resolution. The Dionex CarboPac SA10 column can analyze six common sugars in food samples in only 10 min, or eight common biofuel mono- and disaccharides in less than 10 min.
Fast analysis
High resolution
High capacity
High reproducibility
No derivatization required
The Dionex CarboPac SA10 column is composed of a unique macroporous substrate coated with a strong anion-exchange layer of latex nano beads which provides high capacity and rapid high-resolution separations. The excellent resolution and linearity of this column provides highly accurate, quantitative carbohydrate analysis. Used in conjunction with pulsed amperometric detection (PAD) systems, the Dionex CarboPac SA10 column provides direct quantification of nonderivatized carbohydrates with minimal sample preparation or cleanup, and is capable of separating a wide range of sample concentrations. This column features high reproducibility, and can withstand thousands of injection cycles under high pH conditions.
| CarboPac SA10 Column for Monosaccharide and Disaccharide AnalysisSpecifications | |
|---|---|
| Dimensions | CarboPac SA10 Analytical Column: 4×250 mm and 2 × 250 mm CarboPac SA10 Guard Column: 4 × 50 mm and 2 × 50 mm |
| Maximum operating pressure | 3500 psi (24.5 MPa) |
| Temperature range | 4–60 °C |
| Typical operating conditions | 1700 psi at 1.5 mL/min (standard-bore column) or 0.38 mL/min (microbore column) Recommended flow rate: 1.5 mL/min (standard-bore) or 0.38 mL/min (microbore) |
| Typical Eluents | Potassium hydroxide or sodium hydroxide |
| Resin composition | 6-µm diameter substrate (ethylvinylbenzene 55% crosslinked with divinylbenzene) agglomerated with 55-nm MicroBead difunctional quaternary ammonium ion (4.5% cross-linked). |
| Anion-exchange capacity | Approximately 290 µeq/column (4×250 mm standard-bore column) Approximately 73 µeq/column (2 × 250 mm microbore column) |
| Column construction | PEEK |
Seven minute separation of biofuel sugars using the CarboPac SA10 column
Ten minute separation of food sugars using the CarboPac SA10 column
| Analytical Columns | |
|---|---|
| CarboPac SA10 Analytical Column (4 x 250mm) | 074641 |
| Dionex CarboPac SA10 Analytical Column (2 x 250 mm) | 082322 |
| Guard Columns | |
|---|---|
| CarboPac SA10 Guard Column (4 x 50mm) | 074902 |
| Dionex CarboPac SA10 Guard Column (2 x 50 mm) | 082323 |
