产地:MBI
货号:ER1031 Conditions for 100% Activity
1X Buffer B:
10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2 and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
KspAI is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
Ligation and Recleavage
After 50-fold overdigestion with KspAI, more than 90% of the DNA fragments can be ligated and recut.
ER1031 |
500 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER1031 |
|
ER1032 |
2500 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER1032 |
|