产地：MBI
货号：ER1351 Conditions for 100% Activity
1X Buffer B:
10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2 and 0.1 mg/ml BSA.
Incubate at 65°C. To ensure higher efficiency of digestion, perform the cleavage reaction under paraffin oil.
Ligation and Recleavage
After 50-fold overdigestion with TasI, more than 95% of DNA fragments can be ligated and recut.
Storage Buffer
TasI is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
| ER1351 | 1000 u (10 u/µl) | 1.00 ml | 1.00 ml | ER1351 | |
| ER1352 | 5000 u (10 u/µl) | 2 x 1.00 ml | 1.00 ml | ER1352 | |
产品描述
