产地：MBI 货号：ER1411 Conditions for 100% Activity 1X Buffer B: 10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2 and 0.1 mg/ml BSA. Incubate at 37°C. Ligation and Recleavage After 50-fold overdigestion with ApaI, more than 95% of the DNA fragments can be ligated and recut. Storage Buffer ApaI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 50 mM NaCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
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