| ER1461 | 200 u (10 u/µl) | 1.00 ml | 1.00 ml | ER1461 | |
| ER1462 | 1000 u (10 u/µl) | 1.00 ml | 1.00 ml | ER1462 | |
产地：MBI
货号：ER1461 Conditions for 100% Activity
1X Buffer O:
50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
BshTI is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Digestion of Agarose-embedded DNA
Minimum 20 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
Ligation and Recleavage
After 50-fold overdigestion with BshTI, more than 95% of the DNA fragments can be ligated and recut.
产品描述
