产地：MBI 货号：ER1491 Conditions for 100% Activity 1X Buffer R: 10 mM Tris-HCl (pH 8.5 at 37°C), 10 mM MgCl2, 100 mM KCl and 0.1 mg/ml BSA. Incubate at 30°C. Ligation and Recleavage After 10-fold overdigestion with AloI, approximately 70% of the DNA fragments can be ligated and more than 80% of these can be recut. Storage Buffer AloI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
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