产地:MBI 货号:ER1511 Conditions for 100% Activity 1X Buffer R: 10 mM Tris-HCl (pH 8.5 at 37°C), 10 mM MgCl2, 100 mM KCl and 0.1 mg/ml BSA. Incubate at 37°C. Ligation and Recleavage After 50-fold overdigestion with NmuCI, more than 95% of the DNA fragments can be ligated and recut. Storage Buffer NmuCI is supplied in: 10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.