产地：MBI 货号：ER1831 Conditions for 100% Activity 1X Buffer G: 10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 50 mM NaCl and 0.1 mg/ml BSA. Incubate at 37°C. Ligation and Recleavage After 50-fold overdigestion with Hin1II, more than 95% of the DNA fragments can be ligated and recut. Storage Buffer Hin1II is supplied in: 10 mM potassium phosphate (pH 7.4 at 25°C), 500 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.15% Triton X-100, 0.5 mg/ml BSA and 50% (v/v) glycerol.
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