Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids (1). It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+.
Cat No
Size
Price($)
A1201-200
200 units
75
A1201-500
500 units
155
The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.
Applications
- First- and second-strand synthesis of cDNA.
- Primer extensions and RNA sequencing
- RT-PCR(a). Up to 10μl of an RT reaction containing AMV RT and the supplied AMV RT Reaction Buffer can be added to a 50μl PCR amplification reaction that uses Taq DNA polymerase.
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