BML-SE535-0100 100 µg
Tyrosinase is a copper-containing enzyme that catalyzes four steps in the melanin and catecholamine synthesis pathways. Like tyrosine hydroxylase (EC 1.13.16.2), it can catalyze the formation of L-DOPA in the brain and nervous system. It oxidizes monophenols, but like catecholoxidase (EC 1.10.3.1), it can also oxidize o-diphenols to o-quinones. It is involved in cancer, Parkinson’s, pigmentation diseases, Alzheimer’s and obesity. Much of the research and inhibitor screening has used mushroom tyrosinase.
However, mushroom tyrosinase interacts very differently with substrates and inhibitors compared to the human enzyme. In addition, commercially available mushroom tyrosinase is not always of good purity. Recombinant versions of human tyrosinase amino acids 1-345 or 1-378, also available commercially, are not functional due the lack of critical catalytic and structural residues.
Product Specification
Alternative Name: Monophenol monooxygenase
MW: ~52kDa (SDS-PAGE)
Purity: ~90% (SDS-PAGE)
Activity: One U=100pmol/min at 37°C using 0.5mM L-Tyrosine as substrate in 50mM potassium phosphate, pH 7.3, 2% dimethylformamide, 5mM MBTH (3-methyl-2-benzothiazolinone hydrazone) and monitoring formation of dopachrome-MBTH adduct at 484nm (ε484=22,300 M-1cm-1). NOTE: tyrosinases, in particular mammalian tyrosinase, is inherently a slow enzyme, with typical changes in OD only in the deciOD range. In addition, its substrates and MBTH are very unstable; make stocks fresh and keep sealed in the dark until use.
EC: 1.14.18.1
Source/Host: Produced in E. coli.
Application: Study enzyme kinetics and screen for inhibitors.
Shipping: SHIPPED ON BLUE ICE
Long Term Storage: -20°C
Handling: Avoid freeze/thaw cycles.
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