BML-SL140-0001 1 mg
Sphingosine-1-phosphate (1-SPP) is a putative second messenger that mobilizes calcium from intracellular stores via an IP3 independent pathway, activation of phospholipase D and stimulation of quiescent Swiss 3T3 fibroblast growth. Regulates a variety of cellular responses, including survival, cytoskeletal remodeling, chemotaxis etc. via the activation of cell surface EDG receptors. 1-SPP also promotes angiogenesis.
Product Specification
Formula: C18H38NO5P
MW: 379.5
Purity: ≥98%
Appearance: White solid.
CAS: 26993-30-6
Source/Host: Synthetic.
Solubility: Sparingly soluble in 1:1 ethanol:water. Addition of small amounts of acetic acid may help dissolve this product. Soluble in warm methanol.
For delivery of sphingosine-1-phosphate to cells, prepare a 125μM stock in 4mg/ml BSA as follows: Dissolve sphingosine-1-phosphate (S-1-P) in methanol (0.5mg/ml). This may require boiling (65°C), with occasional replacement of evaporated methanol. Crushing of solid prior to addition of methanol and crushing and stirring during heating is helpful. Aliquot desired amounts of methanol stock to tubes. Evaporate the solvent with a stream of nitrogen, swirling to deposit a thin film on the inside of the tube. Aliquots may be stored at -20 °C at this point or dissolved with enough 4mg/ml BSA (fatty acid free Bovine Serum Albumin in water; 37°C, 30 min. with repeated vortexing) to make 125μM stock.
Shipping: AMBIENT
Long Term Storage: -20°C
Hazard: IRRITANT.
Handling: Packaged under inert gas.
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