BML-UW0545-0100 100 µg
A useful reagent for studying SUMOylation in vitro. Represents a readily detectable alternative to radio-iodinated SUMO-1. Biotinylated SUMO-1 is activated by SUMO E1, a competent substrate for the SUMO E2 conjugating enzyme Ubc9. Can be conjugated to target proteins, in either a SUMO E3 ligase dependent or independent manner, as appropriate. The biotinyl moiety also provides a potential route to affinity purification of conjugated substrates (or thiol ester-linked enzymes) on avidin/streptavidin matrices.
Suggested uses: 1. Ready detection of SUMO-1 modification of specific proteins in vitro. 2. Demonstration that novel proteins are potential targets for SUMOylation under in vitro conditions. 3. Purification of SUMO1-conjugates via streptavidin affinity chromatography.
Product Specification
Alternative Name: Sentrin-1, SMT3C, SMT3H3
MW: ~13.5kDa (based on Bt3-SUMO-1 as predominant form present).
Formulation: Lyophilized solid (from 1:1 acetonitrile:water).
Source/Host: Produced in E. coli.
Reconstitution: In order to ensure full solubility it is recommended that the material be dissolved in 5µL dimethyl sulphoxide (DMSO) prior to dilution with buffer of choice.
Shipping: SHIPPED ON BLUE ICE
Short Term Storage: -20°C
Long Term Storage: -20°C
Use/Stability: Stable for at least 12 months after receipt when stored at -20°C.
Handling: Avoid freeze/thaw cycles.
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