BML-UW0670-0100 100 µg
Product Specification
Purity Detail: Purified by subtractive anion exchange chromatography.
MW: Range
Formulation: Lyophilized.
Source/Host: Synthetic. Ubiquitin was polymerized through Lys48 using E1, UbcH5A, and the C-terminal domain of a specific HECT E3.
Reconstitution: Note: It is recommended that the product be reconstituted at a moderately high protein concentration (>1mg/mL) and in the presence of buffer. If a more dilute reagent is required, the inclusion of an inert carrier protein (e.g. ovalbumin at 0.5-1.0mg/mL) in the storage buffer is recommended in order to minimise loss of the reagent due to absorption.
Application: For use in deubiquitinylating enzyme assays and polyubiquitin binding studies.
Suggested uses:
1. Deubiquitinylating enzyme substrates (general/linkage specific).
2. Investigation of polyubiquitin chain recognition by and interaction with ubiquitin binding proteins.
Shipping: SHIPPED ON BLUE ICE
Short Term Storage: -20°C
Long Term Storage: -20°C
Use/Stability: Store unopened vial at -20°C until required for use.
Handling: Avoid freeze/thaw cycles.
Miscellaneous/General: Polyubiquitin chains, linked through specific lysine residues, are useful tools for investigating the specificity and reactivity of deubiquitinylating enzymes (DUBs) and the recognition and interaction of polyubiquitin modified proteins with ubiquitin binding domain (UBDs) containing proteins.
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