Standard methods for the enumeration of heterotrophic bacteria in water have traditionally used nutritionally rich media, such as Plate Count Agar, with incubation at 35°C1. Organisms isolated under these conditions may represent only a small percentage of the bacteria present in the sample2.
R2A Agar developed by Reasoner and Geldreich2 is a nutritionally reduced medium. It was demonstrated that using this medium and incubating for longer at lower temperatures enhanced the recovery of stressed and chlorine damaged bacteria from treated waters resulting in higher, more realistic bacterial counts.
Storage conditions and Shelf life
R2A Agar should be stored tightly capped in the original container at 10-30°C. When stored as directed the medium will remain stable until the expiry date printed on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.
Directions
Suspend 18.1g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well and pour into sterile Petri dishes.
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