FastAP™ Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA and nucleotides. This enzyme also removes phosphate groups from proteins.
FastAP™ is a novel alkaline phosphatase, which is active in all Fermentas restriction enzyme buffers as well as in PCR buffers. It dephosphorylates all types of DNA ends (blunt, 5'- and 3'-overhangs) in 10 min at 37°C. The enzyme is inactivated in 5 min at 75°C, see Fig.1. Therefore, removal of alkaline phosphatase is not required prior to ligation.
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