Glycosylase mediated single nucleotide polymorphism detection (GMPD) (3).
Site-directed mutagenesis (4).
As a probe for protein-DNA interaction studies (5).
SNP genotyping.
Cloning of PCR products (6).
Generation of single strand overhangs of PCR products and cDNA.
Description
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA, see Fig.1. Shows no activity on RNA (1).
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