First strand cDNA synthesis for RT-PCR and real-time RT-PCR.
Synthesis of cDNA for cloning and expression.
Generation of labeled cDNA probes for microarrays.
DNA labeling (3).
Analysis of RNA by primer extension (3).
Description
RevertAid™ Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase (1, 2).
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