产地：MBI
货号：ER0592 Conditions for 100% Activity
1X Buffer O:
50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
NotI is supplied in:
20 mM Tris-HCl (pH 7.8 at 25°C), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.02% Triton X-100, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 50-fold overdigestion with NotI, more than 95% of DNA fragments can be ligated and recut.
Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded Adenovirus-2 DNA in 16 hours.
ER0591 |
300 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER0591 |
|
ER0592 |
1500 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER0592 |
|
ER0593 |
HC, 1500 u (50 u/µl) |
1.00 ml |
1.00 ml |
ER0593 |
|
ER0595 |
500 u (10 u/µl) |
1.00 ml |
1.00 ml |
ER0595 |
产品描述
