Acetyl-CoA carboxylase 2 Phospho(pS221)(ACACB)antibody
Cat.#: 2666-1
Rabbit Monoclonal Antibody

Clone ID: EPR3143Y
Swiss Prot: O00763
Mol Weight: 280kDa
Size: 100ul

Description
Acetyl-CoA carboxylase 2 (ACC2) is a biotin-dependent enzyme that is involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. ACC2 is predominantly expressed in the heart, skeletal muscles and liver (1). It catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase and carboxyltransferase. ACC2 is throught to control fatty acid oxidation by the means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I (CPT-1), the rate-limiting step in fatty acid uptake and oxidation by mitochondria (2). The activity of ACC2 is controlled by reversible phosphorylation. Ser219 and Ser221 were found to be critical for the phosphorylation and subsequent inactivation of ACC2 (3).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:100
IP: 1:10

Species Reactivity
Human

Products Data

 

Western blot analysis on HeLa cell lysates using anti-Phospho-ACC2 (pS221) RabMAb (cat. #2666-1), 1:5000 dilution. Cells were either (A) untreated (B) treated with calyculin A.
Immunohistochemical analysis of paraffin-embedded human muscles using anti-ACACB RabMAb (cat. # 2666-1).

Specificity
A synthetic peptide corresponding to residues surrounding serine 221 of human ACC2 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ACACB antibody, ACC2 antibody, ACCB antibody, HACC275 antibody, Biotin carboxylase antibody, ACC-beta antibody

Description References
1. Ha J, et al. Proc Natl Acad Sci 93(21):11466-70, 1996 2. Abu-Elheiga LA et al. J Biol Chem 272:10669-77, 1997 3. Tong L et al. Cell Mol Life Sci 62(16) :1784-803, 2005