Actin(ACTC1)antibody
Cat.#: 1844-1
Rabbit Monoclonal Antibody
Clone ID: EP184E
Swiss Prot: P68032
Mol Weight: 42kDa
Size: 100ul

Description
Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).

Recommended Applications
WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 200
ICC: 1:250 - 500
IP: 1:20
FC: 1:20

Species Reactivity
Human, Mouse, Rat

For Mouse, this antibody is recommended for WB only
Rat cross reactivity tested by western blot only

Products Data

 

A. Western blot analysis on A431 cell lysate using anti-Actin RabMAb (cat. # 1844-1), dilution 1:1,000
B. Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin RabMAb (cat. # 1844-1).
C. Immunohistochemical analysis of paraffin-embedded human skeletal muscle using anti-Actin (Pan) RabMAb (Cat# 1844-1).
 
D. Immunofluorescent staining of HeLa cells using anti-Actin RabMAb (cat. # 1844-1).

Specificity
A synthetic peptide corresponding to residues near the N-terminus of human Actin was used as an immunogen. This Actin antibody recognizes all muscle actins.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ACTC1 antibody, ACTC antibody, ASD5 antibody, CMD1R antibody, CMH11 antibody, LVNC4 antibody, Actin, alpha cardiac muscle 1 antibody, Alpha-cardiac actin antibody

Description References
1. Herman, I.M. Curr. Opin. Cell Biol. 5: 48–55, 1993. 2. Bailly, M., et al. Curr Biol. 11: 620–5, 2001. 3. Lim, Y.P., et al. Clin. Cancer Res. 10: 3980–3987, 2004. 4. Olson, T.M., et al. Science 280: 750–2, 1998.

Product References
1. Bhattacharya R, Gonzalez AM, DeBiase PJ, et al, Recruitment of vimentin to the cell surface by β3 integrin and plectin mediates adhesion strength ,J. Cell Sci.122 (2009) 1390 - 1400
2. Wang JJ, Hu HQ, Wang S, et al, The important role of actinin-like protein (AcnA) in cytokinesis and apical dominance of hyphal cells in Aspergillus nidulans ,Microbiology 155 (2009) 2714 - 2725
3. Bhattacharya R, Gonzalez AM, Debiase PJ, et al, Recruitment of vimentin to the cell surface by β3 integrin and plectin mediates adhesion strength ,J Cell Sci 122 (2009) 1390-1400