AGR2 antibody
Cat.#: 2574-1
Rabbit Monoclonal Antibody
Clone ID: EPR3278
Swiss Prot: O95994
Mol Weight: 17kDa
Size: 100ul

Description
The anterior gradient protein-2 (AGR2) is a cancer cell marker specifically up-regulated in response to depletion of serum and oxygen. It has been identified as a tumor marker in primary and secondary cancer lesions, and as a marker for detection of circulating tumor cells. Elevated levels of AGR2 are known to increase the metastatic potential of cancer cells (1). Specifically, AGR2 may serve as a useful molecular marker and/or potential therapeutic target for hormone-responsive breast tumours (2). AGR2 expression promotes tumor growth in esophageal adenocarcinoma cells and intestinal cells of secretory lineage, suggesting that AGR2 may be important for the growth and development of the intestine as well as esophageal adenocarcinomas (3).

Recommended Applications
WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:5,000 - 10,000
IHC: 1:250 - 500
ICC: 1:100 - 250
IP: 1:20
FC: 1:20

Species Reactivity
Human

Cross reactivity determined by western blot only.

Products Data

 

 
A. Western blot analysis on (A) HT-29 and (B) MCF-7 cell lysates using anti-AGR2 RabMAb (cat. #2574-1), dilution 1:10,000. 
B.Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-AGR2 RabMAb (cat. #2574-1). 
C.Immunohistochemical analysis of paraffin-embedded human endometrium adenocarcinoma using anti-AGR2 RabMAb (cat. #2574-1).

Specificity
A synthetic peptide corresponding to residues in human AGR2 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AGR2 antibody, AG2 antibody, GOB-4 antibody, HAG-2 antibody, PDIA17 antibody, XAG-2 antibody, Anterior gradient protein 2 homolog antibody, HPC8 antibody, Secreted cement gland protein XAG-2 homolog antibody

Description References
1. Zweitzig DR, et al. Mol Cell Biochem. 306(1-2):255-60, 2007
2. Fletcher GC, et al. Br J Cancer 88(4):579-85, 2003
3. Wang Z, et al. Cancer Res. 68(2):492-7, 2008