Akt1 Phospho(pS473)(AKT1)antibody
Cat.#: 2118-1
Rabbit Monoclonal Antibody
Clone ID: EP2109Y
Swiss Prot: P31749
Mol Weight: 60kDa
Size: 100ul

Description
Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBa isoforms have been identified.

Recommended Applications
WB, IHC, ICC

Applications and Recommended Dilution Factors
WB: 1:1000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250

Species Reactivity
Human, Mouse

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data  

 

A. Immunohistochemical analysis of untreated cervical carcinoma using anti-Phospho-Akt1 (pS473) RabMAb (cat. # 2118-1).
B. Immunohistochemical analysis of Phosphatase- treated cervical carcinoma using anti-Phospho-Akt1 (pS473) RabMAb (cat. # 2118-1).
C. Western blot analysis on 3T3 cell lysates using anti-Phospho-Akt1 (pS473) RabMAb (cat. #2118-1), 1:10,000 dilution. Cells were either (A) untreated or (B) treated with PDGF.

Specificity
A phospho specific peptide corresponding to residues surrounding Ser473 of human Akt1 was used as immunogen. The antibody detects Akt1 phosphorylated at Serine 473.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AKT1 antibody, AKT antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, RAC-alpha serine/threonine-protein kinase antibody, Proto-oncogene c-Akt antibody, RAC-PK-alpha antibody

Description References
1. Burgering, B.M. and P.J. Coffer.Nature 376: 599
2. Cardone, M.H., et al. Science 282: 1318
3. Hajduch, E., et al. FEBS Lett. 492: 199
4. Cross, D.A., et al. Nature 378: 785-789 (1995).

Product References
1. Renner O, Blanco-Aparicio C, Grassow M, et al, Activation of Phosphatidylinositol 3-Kinase by Membrane Localization of p110α Predisposes Mammary Glands to Neoplastic Transformation ,Cancer Res 68 (2008) 9643-9653
[Application: IHC]
2. Blanco-Aparicio C, Cañamero M, Cecilia Y, et al, Exploring the Gain of Function Contribution of AKT to Mammary Tumorigenesis in Mouse Models ,PLoS One 5 (2010) e9305
[Application: IHC]
3. Pommier AJ, Alves G, Viennois E, et al, Liver X Receptor activation downregulates AKT survival signaling in lipid rafts and induces apoptosis of prostate cancer cells ,Oncogene 29 (2010) 2712-2723
[Application: ICC]
4. Kunoh T, Noda T, Koseki K, et al, A Novel Human Dynactin-Associated Protein, dynAP, Promotes Activation of Akt, and Ergosterol-Related Compounds Induce dynAP-Dependent Apoptosis of Human Cancer Cells ,Mol Cancer Ther 9 (2010) 2934-2942