Annexin V(ANXA5)antibody
Cat.#: 3225-1
Rabbit Monoclonal Antibody
Clone ID: EPR3979
Swiss Prot: P08758
Mol Weight: 36kDa
Size: 100ul

Description
Annexin V (ANV) is a calcium-dependent glycoprotein with a potent anticoagulant capacity in vitro, mainly as a result of its negatively charged membrane phospholipids, and capable of inhibiting the prothrombinase and Tensa complexes to reduce plaque adhesion and aggregation. Circulating ANV can be released from the cells of the vascular wall (endothelial cells, smooth muscles cells) or from secretor cells of the spleen and liver. Once it is in the plasma, it binds to blood cells (platelets and erythrocytes) or to endothelial cells (1). Annexin V is also a Ca(2+) dependent membrane-binding protein that forms voltage-dependent Ca2+ channels in phospholipid bilayers (2). Structural features suggest that Annexin V attaches with its convex face to membrane by specific calcium mediated interactions with at least three phospholipids. The adjacent membrane bilayer may thus become locally disordered and permeable to allow calcium inflow through the central polar channel of the molecule.

Recommended Applications
WB, IHC, ICC

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250

Species Reactivity
Human

Species cross-reactivity is based on WB analysis

Products Data 

 

Western blot analysis on (A) HeLa, (B) JAR, (C) fetal kidney lysates using anti-Annexin V RabMAb (cat. #3225-1).
Immunohistochemical analysis of paraffin-embedded human gastric tissue using anti-Annexin V RabMAb (cat. #3225-1).

Specificity
A synthetic peptide corresponding to residues in human Annexin V was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ANXA5 antibody, ANX5 antibody, ENX2 antibody, PP4 antibody, Annexin A5 antibody, Thromboplastin inhibitor antibody, Vascular anticoagulant-alpha antibody

Description References
1. Shjaie M, et al. Thromb J, 21;7:13, 2009
2. Demange P, et al. Trands Biochem Sci 19(7):272-2, 1994