Apolipoprotein A1(apo A1)(APOA1)antibody
Cat.#: 1920-1
Rabbit Monoclonal Antibody
Clone ID: EP1368Y
Swiss Prot: P02647
Mol Weight: 28kDa
Size: 100ul

Description
Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:60

Species Reactivity
Human

Cross reactivity determined by western blot only.

Products Data  

 

A. Western blot analysis on fetal liver lysate using anti-Apo A1 RabMAb (cat. # 1920-1).
B. Immunohistochemical staining of paraffin-embedded human liver using anti-Apo-A1RabMAb (cat. #1920-1).
C. Immunofluorescent staining of HEPG2 cells using anti-Apo- A1 RabMAb (cat.#1920-1).

Specificity
A synthetic peptide corresponding to residues near the N-terminus of human Apo A1 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
APOA1 antibody, MGC117399 antibody, Apolipoprotein A-I(1-242) antibody, Apolipoprotein A1 antibody

Description References
1. Shoulders CC, et al. Nucleic Acids Res. 11(9):2827-37 1983.
2. Brewer HB, et al. Biochem Biophys Res Commun 113(2):626-32, 1983.
3. Baker HN, et al. J Biol Chem 250(7):2725-38, 1975.