ABI1 Phospho(pY435)antibody
Cat.#: 3244-1
Rabbit Monoclonal Antibody
Clone ID: EP3954
Swiss Prot: Q8IZP0
Mol Weight: 55kDa
Size: 100ul

Description
ABI1 has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, this protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling (1). In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. It regulates ABL1/c-Abl-mediated phosphorylation of MENA, and widely expressed, with highest expression in brain (2).

Recommended Applications
WB, ICC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:10,000 - 50,000
ICC: 1:250 - 500
IP: 1:10 -100
FC: 1:10 - 100

Species Reactivity
Human

Species cross-reactivity is based on WB analysis

Products Data

Western blot analysis on Jurkat cell lysates using anti-Phospho-ABI1 (pY435) RabMAb (cat. #3244-1). Cells were either (A) untreated (B) treated with Pervanadate.

Specificity
A phospho specific peptide corresponding to residues surrounding tyrosine 435 of human ABI1 was used as an immunogen. This antibody only detects ABI1 phosphorylated at tyrosine 435.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ABI1 antibody, ABI-1 antibody, E3B1 antibody, NAP1BP antibody, SSH3BP antibody, SSH3BP1 antibody, Abl interactor 1 antibody, Spectrin SH3 domain-binding protein 1 antibody, e3B1 antibody

Description References
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1; 33: D54-8.
2. The UniProt Consortium. The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195 (2010)