Alpha-Actinin 3(ACTN3)antibody
Cat.#: 2316-1
Rabbit Monoclonal Antibody
Clone ID: EP2531Y
Swiss Prot: Q08043
Mol Weight: 100kDa
Size: 100ul

Description
The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibers. Absence of alpha-actinin-3 is not associated with an obvious disease phenotype, raising the possibility that ACTN3 is functionally redundant in humans, and that alpha-actinin-2 is able to compensate for alpha-actinin-3 deficiency (1). Alpha-Actinin-3 is specifically expressed in fast-twitch myofibers responsible for generating force at high velocity. The absence of a disease phenotype secondary to alpha-actinin-3 deficiency is likely due to compensation by the homologous protein, alpha-actinin-2. However, the high degree of evolutionary conservation of ACTN3 suggests function(s) independent of ACTN2. Highly significant associations between ACTN3 genotype and athletic performance have been demonstrated in both male and female athletes.

Recommended Applications
WB, IHC, ICC, FC

Applications and Recommended Dilution Factors
WB: 1:5,000-10,000
IHC: 1:100 - 250
ICC: 1:100 - 250
FC: 1:200

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

 

Western blot analysis on human skeletal muscle lysate using anti-alpha-actinin 3 RabMAb (cat. # 2316-1), dilution 1:10,000.
Immunohistochemical analysis of paraffin-embedded human muscles using anti-Alpha-Actinin-3 RabMAb (cat. #2316-1).

Specificity
A synthetic peptide corresponding to residues on the N-terminus of human alpha-actinin 3 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ACTN3 antibody, MGC117002 antibody, MGC117005 antibody, Alpha-actinin-3 antibody, Alpha-actinin skeletal muscle isoform 3 antibody, F-actin cross-linking protein antibody

Description References
1. Mills M, et al. Hum Mol Genet 10(13):1335-46, 2001 2. Yang N, et al. Am J Hum Genet 73(3):627-31, 2003