ATF-1(ATF1)antibody
Cat.#: 3183-1
Rabbit Monoclonal Antibody
Clone ID: EPR1590(2)
Swiss Prot: P18846
Mol Weight: 36kDa
Size: 100ul

Description
ATF-1 is a cAMP-responsive enhancer (CRE) binding protein that is expressed in a wide variety of cell types and is capable of dimerizing with CREB. ATF-1 contains a consensus phosphorylation site for PKA and shares extensive homology with CREB in the region surrounding and carboxyl-terminal to the PKA site (1). Epidermal growth factor induction of c-jun expression requires ATF-1 and MEF2 sites in the c-jun promoter. Two MAPK pathways, ERK and JNK, are required for EGF-induced c-jun expression and the ERK pathway acts through downstream phosphorylation of ATF-1 (2).

Recommended Applications
WB, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IP: 1:10 - 100

Species Reactivity
Human

Species cross-reactivity is based on WB analysis

Products Data

 
Western blot analysis on (A) K562, (B) HeLa, and (C) HepG2 cell lysates using anti-ATF-1 RabMAb (cat. #3183-1).

Specificity
A synthetic peptide corresponding to residues in C-terminus of human ATF-1 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ATF1 antibody, EWS-ATF1 antibody, FUS/ATF-1 antibody, TREB36 antibody, Cyclic AMP-dependent transcription factor ATF-1 antibody, Activating transcription factor 1 antibody, Protein TREB36 antibody

Description References
1. Rehfuss RP, et al J Biol Chem 266(28):18431-4, 1991
2. Gupta P et al. J Biol Chem 277(52):50550-6, 2002