ATF-2(C-term)(ATF2)antibody
Cat.#: 1489-1
Rabbit Monoclonal Antibody
Clone ID: E243
Swiss Prot: P15336
Mol Weight: 70kDa
Size: 100ul

Description
ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:250-500
ICC: 1:250-500
IP: 1:60

Species Reactivity
Human

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

 

A. Western blot analysis on HeLa cell lysate using anti-ATF-2 (C-term) RabMAb (cat. #1489-1); dilution 1:10,000.
B. Immunohistochemical analysis of paraffin-embedded breast carcinoma using anti-ATF2 (C-term) RabMAb (cat. #1489-1).

Specificity
A synthetic peptide corresponding to residues in C-terminus of human ATF2 was used as immunogen. The antibody does not cross-react with other ATF family members .

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ATF2 antibody, CRE-BP1 antibody, CREB2 antibody, HB16 antibody, MGC111558 antibody, TREB7 antibody, Cyclic AMP-dependent transcription factor ATF-2 antibody, HB16 antibody, cAMP response element-binding protein CRE-BP1 antibody

Description References
1. Maekawa, T., et al. Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain. EMBO J. 8: 2023
2. Gupta, S., et al. Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science 267: 389
3. van Dam, H., et al. ATF-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotoxic agents. EMBO J. 14: 1798
4. Livingstone, C., et al. ATF-2 contains a phosphorylation-dependent transcriptional activation domain. EMBO J. 14: 1785