A-RAF Antibody (ARAF1) (Center)
Cat.#: T2762
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P10398
Mol Weight: 68kDa
Size: 100ug

Description
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Recommended Applications
WB, IHC, ELISA

Applications and Recommended Dilution Factors
WB: 1:100~500
IHC: 1:50~100
ELISA: 1:1,000

Species Reactivity
Human

Products Data 

 

Western blot analysis of hARAF1-H270 in MCF7 cell line lysates (35ug/lane). ARAF1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Specificity
This A-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263~292 amino acids from the center region of human ARAF1.

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Alternative Names
ARAF antibody, A-RAF antibody, ARAF1 antibody, PKS2 antibody, RAFA1 antibody, Serine/threonine-protein kinase A-Raf antibody, Proto-oncogene A-Raf antibody, Proto-oncogene A-Raf-1 antibody, Proto-oncogene Pks antibody

Description References
Wu, X., et al., J. Biol. Chem. 271(6):3265-3271 (1996).
Lee, J.E., et al., Genomics 20(1):43-55 (1994).
Popescu, N.C., et al., Oncogene 4(4):517-519 (1989).
Beck, T.W., et al., Nucleic Acids Res. 15(2):595-609 (1987).
Yin, X.L., et al., Mol. Cell. Biochem. 231 (1-2), 69-74 (2002).