产地：usa
货号：L8754
Lectin from Phaseolus vulgaris (red kidney bean) 外源凝集素(菜豆)
别名: PHA, 植物血球凝集素(菜豆)
Analysis Note
Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
Biochem/physiol Actions
PHA 由两个分子种类构成，一种是红细胞凝集素 (PHA-E)，具有低促有丝分裂活性和高红细胞凝集活性，另一种是白细胞凝集素 (PHA-L)，具有高促有丝分裂和白细胞凝集活性，但红细胞凝集活性极低。1,2
PHA-E 不具有血型特异性，但凝集作用受某些寡糖抑制。3PHA-P 是在分离和纯化红细胞凝集素和白细胞凝集素前 PHA 的蛋白质形式。4PHA-M 为粘蛋白形式。4由相应的已纯化凝集素制备偶联物。
相关类型           Phaseolus vulgaris (Red kidney bean), Bioactive Small Molecules, Cell Biology, Glycan Recognizing Proteins, L, Lectins
form               lyophilized powder
potency            <62.5 μg per mL agglutination activity
                   <10 μg/mL mitogenic activity
total impurities   salt, free
storage temp.      2-8°C
Lectin from Phaseolus vulgaris (red kidney bean) 外源凝集素(菜豆)