产地：usa
货号：L2500
CAS号: 9001-60-9
名称：L-Lactic Dehydrogenase 来源于兔肌肉
L-Lactic Dehydrogenase from rabbit muscle
别名: (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD
Unit Definition
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
Physical form
Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0
General description
Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .
相关类型 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Application Index, Diagnostic and Analytical Enzymes, Enzyme Class Index,
Enzymes, Inhibitors, and Substrates, General Metabolic Enzymes, General Metabolic Enzymes I-Z, Glycolysis, Lactic Dehydrogenase, Metabolic Pathways, Metabolomics, Plasma & Blood Proteins, Plasma, Blood, and Related Proteins and Reagents
type Type II
form ammonium sulfate suspension
foreign activity pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%
storage temp. 2-8°C