产地:Biomol
货号:BML-AK740-0001
规格:Kit
保存温度:+4°C
名称:Proteasome 20S Assay Kit
Product Specification
Quantity: 96 assays
Kit/Set Contains: Proteasome 20S (human) (purified) (Prod. No. BML-PW8720)Suc-Leu-Leu-Val-Tyr-AMC (Prod. No. BML-P802)Proteasome Assay Buffer (Prod. No. BML-KI340)Sodium Dodecyl Sulfate (Prod. No. BML-KI341)Epoxomicin (Prod. No. BML-PI127)AMC (7-Amino-4-methylcoumarin) Calibration Standard (Prod. No. BML-KI144)1/2 Volume White Microplates (Prod. No. BML-KI571)
Shipping: SHIPPED ON BLUE ICE
Long Term Storage: +4°C
Handling: Do not freeze.
A QuantiZymeTM Assay System - DO NOT FREEZE. Fluorogenic, non-radioactive assay designed to measure chymotrypsin-like protease activity of purified 20S proteasome. This 96-well assay is useful for screening inhibitors and modulators of the 20S proteasome. The kit includes purified, human erythrocyte 20S proteasome (20µg). The Suc-LLVY-AMC fluorogenic peptide substrate supplied with this kit is a proteasome substrate commonly used for measuring chymotrypsin-like peptidase activity. The detection of proteolytic activity is based on the release of free AMC fluorophore and offers the following advantages: non-radioactive, convenient real-time kinetics of substrate cleavage, and excellent sensitivity.
A proteasome inhibitor, epoxomicin, is included as a test inhibitor for screening purposes.
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