For isolation and enrichment of SUMO-3 interacting proteins. SUMO-3 agarose may have considerable utility for the capture/pull down of SUMO-3 interacting partners, including processing/signalling pathway proteins and SUMO-3 modification substrates, from cell lysates and extracts by affinity binding. The immobilized protein retains the exposed C-terminal diglycine required for SUMO-3 modification and therefore may be suitable for in vitro SUMOylation. Captured proteins can be eluted readily for identification, analysis or subsequent biochemical reaction.
Product Specification
Source/Host: Human recombinant SUMO-3 coupled to agarose at 0.5mg protein per ml settled resin.
Application: Capture and analysis of SUMO-3 interacting proteins from cell/tissue lysates. Isolation of specific SUMO-3 interacting proteins for use in subsequent biochemical experiments. Identification of novel SUMO-3 interacting partners. Identification of potential SUMOylation substrates through covalent addition to SUMO-3 target proteins.
Long Term Storage: +4°C