The Sulforhodamine Multi-Caspase Activity Kit detects apoptosis through the covalent binding of a red fluorescent probe to active caspase enzymes in living cells. The sulforhodamine-labeled caspase inhibitor probe (SR-VAD-FMK; excitation at 550 nm and emission at 595 nm), comprising a sulforhodamine derivative of valylalanylaspartic acid fluoromethyl ketone (VAD-FMK), covalently binds to the reactive cysteine (Cys 285) on the large subunit of active caspase heterodimers. This probe is both cell permeable and non-cytotoxic during the time of the labeling procedure and allows detection of apoptosis by fluorescence microscopy, flow cytometry, and 96-well-plate based fluorometry.
Use of the fluorescence microscope provides a qualitative view of the level of apoptosis induced as compared to the use of a fluorometer or a flow cytometer which will generate quantitative data. One kit includes enough reagent to test 100 samples. The Carboxyfluorescein Multi-Caspase Activity Kit for Apoptosis Detection is a related kit that contains a carboxyfluoroscein-labeled probe (Ex: 490 nm, Em: 520 nm).
Product Specification
Kit/Set Contains: SR-VAD-FMK Reagent, lyophilized, 10X Wash Buffer, Fixative, Hoechst Stain.
Shipping: SHIPPED ON BLUE ICE
Long Term Storage: -20°C