Fluorogenic substrate for ECE-1 and ECE-2, ACE1 and ACE2, neprilysin, MMP-2 and MMP-9, thimet oligopeptidase, as well as cathepsin A, cathepsin X/Z, BACE, and insulinase, but not MMP-1.Mca fluorescence is quenched by the Dnp group until cleavage separates them. Ex.: 328nm, Em.: 393nm, although the following Ex/Em have also been used: 320-340/400-420. This substrate is useful for inhibitor screening and kinetic analysis.
Product Specification
MW: 1388.5
Purity: 98%
Sequence: Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH[Mca=(7-methoxycoumarin-4-yl)acetyl;Dnp=2,4-dinitrophenyl]
Shipping: SHIPPED ON BLUE ICE
Long Term Storage: -20°C
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