产地：Biomol 
货号：BML-P303-0005
Much more sensitive substrate for leukocyte proteinase 3 (PR-3, myeloblastin) (Kcat/km=1.06 x 106 M-1s-1, Km=63 µM) than MeOSuc-AAPV-pNA (Chromogenic Substrate) (Prod. No. BML-P213) (Kcat/km=1.06 x 103 M-1s-1, Km=470 µM). Also cleaved by neutrophil elastase (Kcat/km=9.2 x 106 M-1s-1, Km=2.2 µM), granzyme H, cathepsin G, chymotrypsin, and rat MCPI and II.Typical assay conditions: substrate concentration 88-250 µM; enzyme concentration 0.2-20 nM; buffer [100 mM HEPES pH 7.5, 500 mM NaCl, 10% DMSO]; with 170 µM either 5,5’-dithiobis(2-nitrobenzoic acid) (DTNB) or 4,4’-dithiobis(pyridine) (from 15 mM DMSO stocks); 25°C.
Hydrolysis of substrate can be detected at 412 nm for DTNB (ε405=13,600) or 324 nm for 4,4’-dithiobis(pyridine) (ε405=19,800).
Product Specification
Formula: C23H35N3O5S
MW: 465.2
Purity: ≥95% (HPLC)
Appearance: White to off-white powder.
Sequence: t-butyloxycarbonyl-Ala-Ala-Nva-thiobenzyl ester
Peptide Content: 60-80%
Solubility: Soluble in methanol or DMSO (≥15mM).
Shipping: SHIPPED ON BLUE ICE
Long Term Storage: -20°C
Use/Stability: Stock solutions in DMSO are stable for up to 3 months when stored at -20°C.
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