服务地域：全国
产品参数：货号：212215 ；规格：pBC SK(+) Phagemid...
属性：产地：美国； 品牌：安捷伦 ；货期：现货
Xho I/Gigapack III 载体试剂盒
SuperCos I 载体
pBlueScript II 载体
Lambda ZAP 载体试剂盒
Lambda ZAP II 载体试剂盒
Lambda gt11 载体试剂盒
产品描述
![](https://www.agilent.com/common/requestQuote.jsp?source=contactus>联系我们</a> </li></ul>
</div>
</header>
</section>
<footer>
<a class="pull-right" href="#"> 返回页首<i class="fa fa-chevron-circle-up"></i></a>
</footer>
</section>
<!-- End of features section from the Consumables Product Asset -->
<!--templateName->/SpecificationsSectionPageletTemplate -->
<!--templateName->/SystemComponentsSectionPageletTemplate -->
<!--Time to load systemComponent section : 21--><!--templateName->/TechnologySectionPageletTemplate -->
<!--templateName->/SideBySideSectionPageletTemplate -->
<script type="text/javascript">
var sections = {"section1":{"template":"SideBySideImageTemplate","templateId":"1405222579069_0","id":"section_1405222579069","name":"pBC Phagemid Vectors","nameId":"1405222579069_1406546460262","assettype":"Section_C"}};
</script>
<script type="text/javascript">
var howItWorksSection = {"section":{"data":{"attributes_list":"id,home_section_title,section_cta_title,analytics_section_title,section_cta_url,section_links,section_sub_text,component_headline,component_desc","id":"1405228393695","idId":"1405228393695_0","home_section_title":"pBC 噬菌粒载体","home_section_titleId":"1405228393695_1406546460262","analytics_section_title":"NoAnalytics","analytics_section_titleId":"1405228393695_1405257770331","assettype":"Section_C","slots":[{"ckeditorAttributes":"slot_basic_desc","attributes_list":"slot_title,slot_basic_desc,slot_digital_image,image_allign,grey_background,slot_digital_imageZoom,slot_video,cta_label,slot_url,display,id,page-url,label","slot_title":"启动子和多接头","slot_titleId":"1405228395920_1406546459576","slot_basic_desc":"<table border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"402\">\r\n\t<colgroup>\r\n\t\t<col width=\"402\" />\r\n\t</colgroup>\r\n\t<tbody>\r\n\t\t<tr height=\"240\">\r\n\t\t\t<td class=\"xl65\" height=\"240\" style=\"height:180.0pt;width:302pt\" width=\"402\"><p>噬菌粒的多接头含 21 个独特的限制性内切酶识别位点。T7 和 T3 RNA 聚合酶启动子可用于体外合成 RNA<font class=\"font5\"></font><font class=\"font0\">。用于转录起始的启动子决定了克隆到多接头中的插入片段的哪条链将被转录。<br /> <br />pBC (+) 和 (–) 可定为两个多接头方向：KS 或 SK。在 KS 方向中，Kpn I 限制性位点最接近 lacZ 启动子，Sac I 限制性位点离 lacZ 启动子最远。而在 SK 方向中，正好相反。</font></p>\r\n\t\t\t\t<p> </p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t</tbody>\r\n</table>\r\n<p> </p>\r\n","slot_basic_descId":"1405228395920_1457965536647","slot_digital_imageZoom":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_image":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_imageId":"1405228395920_1457965621962","image_allign":"Left","image_allignId":"1405228395920_1457965561153","grey_background":"White","grey_backgroundId":"1405228395920_1457965561160","display":"/SideBySideImageTemplate","id":"1405228395920","assettype":"Slots_C"},{"ckeditorAttributes":"slot_basic_desc","attributes_list":"slot_title,slot_basic_desc,slot_digital_image,image_allign,grey_background,slot_digital_imageZoom,slot_video,cta_label,slot_url,display,id,page-url,label","slot_title":"共 131 种氨基酸","slot_titleId":"1405228396182_1406546459576","slot_basic_desc":"<table border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"350\">\r\n\t<colgroup>\r\n\t\t<col width=\"350\" />\r\n\t</colgroup>\r\n\t<tbody>\r\n\t\t<tr height=\"240\">\r\n\t\t\t<td class=\"xl65\" height=\"240\" style=\"height:180.0pt;width:263pt\" width=\"350\"><p>多接头以及 T7 和 T3 RNA 聚合酶启动子序列存在于 lacZ 基因片段的 N 端。从 pBC 质粒中的 MET 序列到 EcoR I 位点有 36 个氨基酸。共有 131 个氨基酸，但被大尺寸多接头中断。在多接头中不含插入片段的 pBC 噬菌粒将在适宜的细菌菌株上生长为蓝色菌落。具有插入片段的 pBC 噬菌粒将在同一菌株上生长为白色菌落，因为插入片段破坏了 lacZ 基因片段的编码区。</p>\r\n\t\t\t\t<p> </p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t</tbody>\r\n</table>\r\n<p> </p>\r\n","slot_basic_descId":"1405228396182_1457965536647","slot_digital_imageZoom":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_image":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_imageId":"1405228396182_1457965621962","image_allign":"Right","image_allignId":"1405228396182_1457965561153","grey_background":"White","grey_backgroundId":"1405228396182_1457965561160","display":"/SideBySideImageTemplate","id":"1405228396182","assettype":"Slots_C"},{"ckeditorAttributes":"slot_basic_desc","attributes_list":"slot_title,slot_basic_desc,slot_digital_image,image_allign,grey_background,slot_digital_imageZoom,slot_video,cta_label,slot_url,display,id,page-url,label","slot_title":"可用于 f1 原点的 (+) 和 (-) 方向","slot_titleId":"1405228396382_1406546459576","slot_basic_desc":"<table border=\"0\" cellpadding=\"0\" cellspacing=\"0\" width=\"341\">\r\n\t<colgroup>\r\n\t\t<col width=\"341\" />\r\n\t</colgroup>\r\n\t<tbody>\r\n\t\t<tr height=\"240\">\r\n\t\t\t<td class=\"xl65\" height=\"240\" style=\"height:180.0pt;width:256pt\" width=\"341\"><p>BssH II 位点这种罕见的六碱基切割位点可以使插入片段和 T 噬菌体 RNA 启动子被切除并用于基因定位。<br /> <br />pBC 噬菌粒可以作为单链 DNA 被拯救，并含有一个 454 个核苷酸的丝状 f1 噬菌体基因间区。f1 基因间区的 (+) 和 (–) 方向可通过辅助噬菌体拯救正义或反义 ssDNA。该 ssDNA 可用于双脱氧核苷酸测序或位点特异性突变。</p>\r\n\t\t\t\t<p> </p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t</tbody>\r\n</table>\r\n<p> </p>\r\n","slot_basic_descId":"1405228396382_1457965536647","slot_digital_imageZoom":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_image":"/cs/publishingimages/SideBySide_GenericDNA.jpg","slot_digital_imageId":"1405228396382_1457965621962","image_allign":"Left","image_allignId":"1405228396382_1457965561153","grey_background":"White","grey_backgroundId":"1405228396382_1457965561160","display":"/SideBySideImageTemplate","id":"1405228396382","assettype":"Slots_C"}]}}};
</script>
<section class="item-collection clear pdpSections active operation-block-section">
<header class="col-full operation-block-header">
<div id="howitworks" class="heading" role="heading" aria-level="3">工作原理</div>
</header>
<section class="item col-full operation-block" data-section="pdp:operation">
<div class="media-body" >
<how-it-works></how-it-works>
<script>
var x=document.querySelector(){kind=link}