存储条件：−20°C
General description
The monoclonal Anti-FLAG BioM2 mouse antibody is covalently attached to biotin by hydrazide linkage. The antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus or C-terminus of FLAG fusion porteins.
Preparation Note
Dilute antibody in TBS (.05M Tris, pH7.4, with .15M NaCl) to a final concentration of 1-10μg/mL.
Physical form
Solution in 50% glycerol, 10 mM sodium phosphate, pH 7.25, containing 150 mM NaCl and 0.02% sodium azide
Application
Biotin-labeled antibody is used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Antibody is suitable for immunofluorescence, western blotting, microscopy applications and for the formation of avidin-biotin complexes.
Browse additional application references in our FLAG
Literature portal.
| biological source | mouse |
| antibody form | purified immunoglobulin |
| clone | M2, monoclonal |
| form | buffered aqueous glycerol solution |
| application(s) | dot blot: suitable (chemiluminescent detection) |
| isotype | IgG1 |
| conjugate | biotin conjugate |
| shipped in | dry ice |
| storage temp. | −20°C |
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