产品描述
The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.
Browse additional application references in our FLAG® Literature portal.
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The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.
FLAG; peptide sequence DYKDDDDK
Purified by affinity chromatography on a column bearing the immunizing peptide.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
| Related Categories | Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, FK-FO, FLAG, FLAG Antibodies, |
| biological source | rabbit |
| antibody form | affinity isolated antibody |
| form | buffered aqueous solution |
| shelf life | 1 mo. at 2 - 8 °C |
| concentration | ~.8 mg/mL |
| application(s) | dot blot: 1-2.5 μg/mL |
| immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate | |
| indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK | |
| western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein | |
| immunogen sequence | DYKDDDDK |
| conjugate | unconjugated |
| shipped in | dry ice |
| storage temp. | −20°C |
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