General description

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control. ^[1]

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

包装

1000, 5000, 10000, 50000 units in glass bottle

Unit Definition

One unit will produce a ΔA₆₀₀ of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Physical form

Lyophilized powder containing Ficoll^® and sodium succinate buffer salts

Application

Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. ^[3] It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. ^[2] It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Biochem/physiol Actions

变溶菌素是一种 N-乙酰胞壁质酶。如溶菌酶一样，它是一种可裂解细菌细胞壁聚合物肽聚糖多糖的 β-N-乙酰胞壁酰-(1→4)-N-乙酰葡糖胺键的胞壁质水解酶。其羧基末端部分与识别和结合唯一的细胞壁聚合物有关。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。

法律信息

Fiberfrax is a registered trademark of Unifrax Corp.

性质

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