产品描述
| 敏感性 | 对热敏感 |
| 溶解性 | Soluble in water (50 mg/ml), ethanol (~1 mg/ml), DMSO (~13 mg/ml), DMF (~14 mg/ml), and PBS, pH 7.2 (~10 mg/ml). |
| 存贮条件 | 储存温度-20°C |
| 应用 | An aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene. |
| 产品介绍 | Puromycin 2HCl 是一种氨基核苷抗菌素，其可作为蛋白质合成的抑制剂。 |
| 生化机理 | Puromycin is an aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene, whose product, puromycin-N-acetyl-transferase, inactivates puromycin via acetylation. This product has been cell culture tested and is recommended for selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles. BACKGROUND: Puromycin is an antibiotic substance produced by the soil actinomycete Streptomyces alboniger which induces apoptosis in cells by interfering with RNA function, leading to inhibition of protein synthesis. Toxic to both eukaryotic and prokaryotic cells. Believed to act as an acyl-tRNA analogue causing premature chain termination, it has been shown to arrest cells in G2/M phase. |
| 别名 | 嘌呤霉素二盐酸盐 ;二氢氯化嘌呤毒素;3'-[α-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride ;Stylomycin hydrochloride |
