熔点 | 190-192°C |
溶解性 | Soluble in water (1M) at 20 °C, ethanol (slightly), ether (slightly), and chloroform (slightly). |
存贮条件 | 室温保存 |
密度 | 1.0500 |
应用 | 生物缓冲剂低温生化工作中推荐buffer。用在制备稳定的底物溶液,用于血清鸟嘌呤酶测定。也是用途非常广泛的一种Buffer,用于酶反应缓冲液,电泳缓冲液,工作浓度从3-100mM。 |
产品介绍 | Bicine is a zwitterionic amino acid buffer, active in the pH 7.6-9.0 range (pKa of 8.26 at 25°C). Recommended buffer for low temperature biochemical work. Bicine is used for the preparation of stable substrate solution for serum guanase determination. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described. |
别名 | N,N-二(2-羟乙基)氨基乙酸;N,N-二羟乙基甘氨酸;二乙醇甘氨酸;二羟乙甘胺酸Bicine;Diethylolglycine;N,N-Di-(Hydroxyethyl) glycine;N,N-Bis(2-hydroxyethyl)glycine |