熔点 | 195°C |
旋光率 | -68.8 ° (C=1, H2O) |
存贮条件 | -20℃充氩保存 |
密度 | 1.5990 |
应用 | 用于检测β-半乳糖苷酶(β-galactosidase)活性,是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物,在410-420nm波长可以检测吸光度。 |
产品介绍 | 溶于水、甲醇,水中溶解度可达15mg/ml。有吸湿性。 |
生化机理 | β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period. |
别名 | ONPG;邻硝基苯-β-D-吡喃半乳糖苷;o-Nitrophenyl β-D-galactopyranoside ONPG |