默克生命科学旗下Millipore子品牌的超滤产品久负盛名，提供不同规格和通量的生物大分子（蛋白/核酸/病毒）、囊泡及纳米材料浓缩、脱盐、换液系列工具，达到操作简便、样本得率高。

Amicon® Ultra超滤管

• 满足从0.5-15mL样本的处理

• 0.5mL/2mL/4mL/15mL四种体积

• 3/10/30/50/100kDa五种截留分子量

UFC9030 Millipore  Amicon® Ultra-15离心式过滤器

Ultracel® Regenerated Cellulose (30kDa MWCO), 15 mL sample volume

别名：Centrifugal concentrator, 30 kDa MWCO, 15 mL sample volume

eCl@ss：36100101

NACRES：NB.24

属性

质量水平

400

物料

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

无菌性

non-sterile

产品线

Amicon®

manufacturer/tradename

Amicon® Ultra

参数

15 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

长度

12.1 cm (4.807 in.)

直径

2.97 cm

过滤面积

7.6 cm2

孔径

30 kDa NMWCO

运输

ambient

说明

一般描述

Amicon® Ultra 15 mL centrifugal devices with Ultracel® 30,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Ovalbumin (45 kDa) at 1 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.

应用

• Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane.

• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.

• Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.

• Concentration of dilute or pre-purified proteins from column eluents.

• Desalting, buffer exchange and protein dialysis.

特点和优势

• Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%.

• Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).

• Heat-sealed membrane minimizes downstream extractables.

• 100% integrity tested for reliable performance.

• Convenient sample monitoring with translucent housing and volume gradations.

• Direct pipettor sample access eliminates processing step to recover concentrate.

• High concentration factors of 80–100X.

外形

色码：透明，带有粉色盖

法律信息

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany