产品描述
ProSwift ConA-1S Affinity Column for Glycan, Glycopeptide and Glycoprotein Enrichment and Purification
The ProSwift ConA-1S affinity monolith column is unsurpassed for fast, highly efficient enrichment and purification of Concanavalin A (Con A) binding glycans, glycopeptides, and glycoproteins from complex samples. The HPLC- compatible ProSwift ConA-1S affinity column maintains the specificity and selectivity for Con A binding glycans and glycoconjugates. The high capacity and ligand density of the ProSwift ConA-1S column facilitates the highly efficient enrichment of samples and can purify up to 2 mg of glycoproteins. The high peak efficiency of the column produces sharp, narrow peaks resulting in low elution volumes of highly concentrated products. The column is reusable, and many enrichments and purifications are possible with minimal loss of capacity.
Features:
• Highly efficient enrichment and purification
• High capacity and ligand density
• High sample recovery
• Low elution volumes
• Fast separations
• HPLC compatible
• Reusable
The ProSwift ConA-1S column, designed for and used in HPLC systems, provides many advantages compared to standard manual methods. These include faster separations, better enrichment and sample recovery, efficient washing capabilities, high peak efficiency and small elution volumes. Other advantages of the ProSwift ConA-1S column used with HPLC systems include automation, reusability, high-throughput capability and more accurate analysis with on-line monitoring.
| ProSwift ConA-1S Affinity Column Specifications | |
|---|---|
| Column Dimension | 5x50 mm |
| Base Matrix Material | Polymethacrylate |
| Surface Ligand | Concanavalin A (Con A) |
| Column protein binding capacity | > 2 mg HRP per column |
| Bound Protein per column | 12-16 mg Con A |
| Ligand density | ~14 mg/ml |
| Bed Height | 49 mm |
| Bed volume | 0.77 mL |
| pH Range | 5 – 8 |
| Maximum Flow rate | Up to 2 mL/min |
| Maximum Pressure | < 2000 psi 8.9 MPa |
| Operating Temperature | < 30 oC |
| Solvent Compatibility | Up to 10% Methanol |
Horseradish peroxidase (HRP) injected at high (2 mg) and low (10 μg) loading on the ProSwift ConA-1S column.
Fast, highly efficient enrichment and purification of a group of fluorescently labeled glycans in under 6 min using the ProSwift ConA-1S column.
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Enrichment and purification of a group of immunodepleated glycoproteins using the ProSwift ConA-1S column, A) followed by analysis of the digested unbound, B) and bound, and C) fractions using RP-LC-MS.
Glycopeptide enrichment using the ProSwift ConA-1S column.
Different glycoforms of chicken ovalbumin separated on the ProSwift ConA-1S column.
| Analytical Columns | |
|---|---|
| ProSwift ConA-1S Affinity Column (5 x 50mm) | 074148 |
