产品描述
AbPac HIC-20 for Monoclonal Antibody (mAb) Analysis
The Thermo Scientific™ MAbPac™ HIC-20 column is a high-resolution, hydrophobic interaction chromatography(HIC) column designed for the separation of mAbs and mAb variants. Its unique proprietary column chemistry provides high resolution, rugged stability, and desired selectivity for the analysis of mAbs and related variants.
The MAbPac HIC-20 column is based on high-purity, spherical, wide-pore (1,000 Å), 5 µm silica particles functionalized with proprietary alkyl amide groups. The advanced surface-bonding technology leads to excellent chemical stability, high resolution, and unique selectivity optimized for mAbs and different from other HIC phases.
Product Highlights
• Optimal selectivity for mAbs and related variants
• Excellent separation for mAb fragments and oxidized variants
• High resolution and high efficiency
• Compatible with both organic solvent and aqueous mobile phase
• Rugged column stability
| MAbPac™HIC-20 Monoclonal Antibody Analysis Column Specifications | |
|---|---|
| Column Dimension | 4.6 × 100 mm 4.6 × 250 mm 4.6 x 10mm guard cartridges |
| Particle Size | 5 μm |
| Bonding Chemistry | Proprietary alkyl amide |
| Silica Substrate | Spherical, high-purity porous silica |
| Pore size | 1,000 Å |
| Flow Rate Range: | 0.5 to 1.0 mL/min. |
| Pressure Limit | 6,000 psi for 4.6 x 100mm column 8,000 psi for 4.6 x 250mm column 6,000 psi for 10mm guard cartridges |
| Temperature Range: | 5 to 60 ºC |
| pH Range | 2.0 - 8.0 |
| Typical buffers: | Mobile phase formula 1 Mobile phase formula 2 |
| Solvent Compatibility: | Compatible with 100% organic solvents |
HIC is a technique for the separation of proteins, including mAbs. The HIC mobile phase usually consists of a salting-out agent, which at high concentration retains the protein by increasing the hydrophobic interaction between the protein and the stationary phase. Bound proteins are eluted by decreasing the salt concentration. HIC has been used as an orthogonal method to size-exclusion chromatography (SEC) and ion-exchange chromatography (IEC) for the characterization of mAb heterogeneity. Analysis of succinimides, antibody fragments, oxidated mAbs, C-terminal lysine modifications, and drug-conjugated mAbs were successfully performed using HIC to monitor the stability, and in some cases the potency, of the drug.
The Thermo Scientific™ MAbPac™ HIC-20 column is ahigh resolution silica column designed for separation ofmonoclonal antibodies (mAbs), mAb fragments and oxidized variants using hydrophobic interaction chromatography (HIC).
| Analytical Columns | |
|---|---|
| MAbPac HIC-20, Analytical 5 µm, 4.6 x 100 mm | 088553 |
| MAbPac HIC-20, Analytical 5 µm, 4.6 x 250 mm | 088554 |
| Guard Columns | |
|---|---|
| MAbPac HIC-20, Guard Cartridges 5 µm, 4.6 x 10 mm (2/pk) | 088555 |
| Acclaim SST Guard Cartridge Holder V-2 | 069580 |
| Acclaim Guard Kit (Holder and coupler) V-2 | 069707 |
| Guard to Analytical Column Coupler V-2 | 074188 |
