DiSC3(5) 产品活性：DiSC3(5) 是一种荧光探针，常用作示踪染料来评估线粒体膜电位。DiSC3(5) 的激发/发射波长最大为 622/670 nm. DiSC3(5) 对线粒体 NAD 相关呼吸系统有抑制作用，IC50 值为 8 μM。DiSC3(5) 在Na+/K+-ATP 酶抑制剂 ouabain 2 的存在下可以诱导埃利希腹水肿瘤细胞的膜超极化。 产品来源： https://www.medchemexpress.cn/disc3-5.html 研究领域：Others 作用靶点：Fluorescent Dye In Vitro: 1. Preparation of DiSC3(5) membrane staining solution: 1.1 Preparation of DMSO or EtOH reserve solution: The reserve solution should be prepared in DMSO or EtOH at 1-5 mM. Note 1) : The unused portion of the reserve solution should be stored at -20 ° C. Avoid repeated freezing/thawing cycles. 1.2 Prepare the working solution: Dilute the reserve solution into a suitable buffer, such as serum-free medium, HBSS or PBS, to make a working solution of 1 to 5 uM. Note 2) : For different cell types and/or experimental conditions, the concentration of the working solution should be determined according to experience. It is recommended to test at concentrations at least in excess of the ten-fold range. 2. Dye the cells into a suspension: 2.1 The suspended cell density in the dye working solution was 1×106/ mL. 2.2 Incubate at 37°C for 2-20 minutes. The culture time depends on the cell type. It is incubated first for 20 minutes and then optimized as needed to obtain even labeling. 2.3 Centrifuge the labeled suspension tubes at 1000 to 1500rpm for 5 minutes. 2.4 Remove the supernatant and gently re-suspend the cells in a preheated (37°C) growth medium. 2.5 Wash twice according to steps 2.3 and 2.4. 3. Staining adherent cells: 3.1 The adherent cells were cultured on a sterile glass cover slide. 3.2 Remove the cover glass from the growth medium and gently drain the excess medium. Place the cover glass in the humidity box. 3.3 Transfer the 100 μL dye working solution to the corner of the cover glass and stir gently until all cells are covered. 3.4 Incubate the cover glass at 37°C for 2-20 minutes. The culture time varies according to cell type. Incubation is started for 20 minutes and then optimized as needed to obtain even labeling. 3.5 Drain the dye working solution and clean the cover glass with growth medium two to three times. For each wash cycle, cover the cells with preheated growth medium, incubate for 5-10 minutes, and then drain the medium. 4. Microscope inspection. 5. Flow cytometry detection: Dis-labeled cells can be analyzed using conventional FL3 flow cytometry detection channels. 相关产品：MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。